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raw huprot protein microarray matrices  (Mendeley Ltd)

 
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    Mendeley Ltd raw huprot protein microarray matrices
    Autoantibody Detection Unveils an Autoreactive Repertoire Enriched in MIS-C Patients (A) Upset plots delineating the number of shared autoantibodies between MIS-C patients, which were at least two-fold enriched when compared with controls for IgG autoantigens in <t>HuProt</t> protein <t>microarray</t> analysis. Upset plots were anchored on autoantibodies that were present in at least one IVIG-treatment-naïve sample (MIS-C 3 and MIS-C 9). Only intersections of 6 or more patients are visualized. (B) Corresponding upset plots for IgA autoantigens. (C) Heatmap of all IgG autoantigens with at least 4-fold enrichment in MIS-C compared to controls, in addition to the selection criteria above. Color intensity corresponds to the log 2 FC expression value relative to the mean of healthy pediatric controls (N=4). Flagged autoantigens were enriched in 5 patients and at least one treatment naive IVIG (MIS-C 3 or MIS-C 9) sample. (D) Corresponding heatmap for IgA autoantigens. (E) Top: validation of protein microarray hits identified by phage immunoprecipitation sequencing (PhIP-seq) for IgG autoantigens. The purple circle and corresponding number indicate the number of autoantigens enriched in the HuProt protein microarray that were also validated by PhIP-seq. Autoantigen peptides were collapsed at the gene level for overlap analyses. Bottom: corresponding overlap for IgA autoantigens. (F) Standard ELISA for CD244 auto-reactivity in MIS-C and healthy control plasma. (G) GSEA (gene set enrichment analysis) analysis of IgG autoantigens in treatment naïve MIS-C patients (N = 2; MIS-C 3 and MIS-C 9) versus age matched healthy controls (N = 4) ranked by t statistic. Dot color intensity corresponds to adjusted p value (FDR) and dot size represents the number of autoantigens found to be enriched in the associated gene set. (H) Corresponding enrichment scores for significantly (FDR<0.05) enriched biological pathways for IgG (regulation of immune response) and IgA (lymphocyte mediated immunity). Benjamini-Hochberg method was used to correct for multiple comparisons. See also <xref ref-type=Figure S5 . " width="250" height="auto" />
    Raw Huprot Protein Microarray Matrices, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/raw huprot protein microarray matrices/product/Mendeley Ltd
    Average 90 stars, based on 1 article reviews
    raw huprot protein microarray matrices - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C)"

    Article Title: Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C)

    Journal: Cell

    doi: 10.1016/j.cell.2020.09.034

    Autoantibody Detection Unveils an Autoreactive Repertoire Enriched in MIS-C Patients (A) Upset plots delineating the number of shared autoantibodies between MIS-C patients, which were at least two-fold enriched when compared with controls for IgG autoantigens in HuProt protein microarray analysis. Upset plots were anchored on autoantibodies that were present in at least one IVIG-treatment-naïve sample (MIS-C 3 and MIS-C 9). Only intersections of 6 or more patients are visualized. (B) Corresponding upset plots for IgA autoantigens. (C) Heatmap of all IgG autoantigens with at least 4-fold enrichment in MIS-C compared to controls, in addition to the selection criteria above. Color intensity corresponds to the log 2 FC expression value relative to the mean of healthy pediatric controls (N=4). Flagged autoantigens were enriched in 5 patients and at least one treatment naive IVIG (MIS-C 3 or MIS-C 9) sample. (D) Corresponding heatmap for IgA autoantigens. (E) Top: validation of protein microarray hits identified by phage immunoprecipitation sequencing (PhIP-seq) for IgG autoantigens. The purple circle and corresponding number indicate the number of autoantigens enriched in the HuProt protein microarray that were also validated by PhIP-seq. Autoantigen peptides were collapsed at the gene level for overlap analyses. Bottom: corresponding overlap for IgA autoantigens. (F) Standard ELISA for CD244 auto-reactivity in MIS-C and healthy control plasma. (G) GSEA (gene set enrichment analysis) analysis of IgG autoantigens in treatment naïve MIS-C patients (N = 2; MIS-C 3 and MIS-C 9) versus age matched healthy controls (N = 4) ranked by t statistic. Dot color intensity corresponds to adjusted p value (FDR) and dot size represents the number of autoantigens found to be enriched in the associated gene set. (H) Corresponding enrichment scores for significantly (FDR<0.05) enriched biological pathways for IgG (regulation of immune response) and IgA (lymphocyte mediated immunity). Benjamini-Hochberg method was used to correct for multiple comparisons. See also <xref ref-type=Figure S5 . " title="... when compared with controls for IgG autoantigens in HuProt protein microarray analysis. Upset plots were anchored on ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Autoantibody Detection Unveils an Autoreactive Repertoire Enriched in MIS-C Patients (A) Upset plots delineating the number of shared autoantibodies between MIS-C patients, which were at least two-fold enriched when compared with controls for IgG autoantigens in HuProt protein microarray analysis. Upset plots were anchored on autoantibodies that were present in at least one IVIG-treatment-naïve sample (MIS-C 3 and MIS-C 9). Only intersections of 6 or more patients are visualized. (B) Corresponding upset plots for IgA autoantigens. (C) Heatmap of all IgG autoantigens with at least 4-fold enrichment in MIS-C compared to controls, in addition to the selection criteria above. Color intensity corresponds to the log 2 FC expression value relative to the mean of healthy pediatric controls (N=4). Flagged autoantigens were enriched in 5 patients and at least one treatment naive IVIG (MIS-C 3 or MIS-C 9) sample. (D) Corresponding heatmap for IgA autoantigens. (E) Top: validation of protein microarray hits identified by phage immunoprecipitation sequencing (PhIP-seq) for IgG autoantigens. The purple circle and corresponding number indicate the number of autoantigens enriched in the HuProt protein microarray that were also validated by PhIP-seq. Autoantigen peptides were collapsed at the gene level for overlap analyses. Bottom: corresponding overlap for IgA autoantigens. (F) Standard ELISA for CD244 auto-reactivity in MIS-C and healthy control plasma. (G) GSEA (gene set enrichment analysis) analysis of IgG autoantigens in treatment naïve MIS-C patients (N = 2; MIS-C 3 and MIS-C 9) versus age matched healthy controls (N = 4) ranked by t statistic. Dot color intensity corresponds to adjusted p value (FDR) and dot size represents the number of autoantigens found to be enriched in the associated gene set. (H) Corresponding enrichment scores for significantly (FDR<0.05) enriched biological pathways for IgG (regulation of immune response) and IgA (lymphocyte mediated immunity). Benjamini-Hochberg method was used to correct for multiple comparisons. See also Figure S5 .

    Techniques Used: Immunopeptidomics, Microarray, Selection, Expressing, Biomarker Discovery, Immunoprecipitation, Sequencing, Enzyme-linked Immunosorbent Assay, Control, Clinical Proteomics


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Saline, Blocking Assay, Staining, Microarray, Software, Immunoprecipitation



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    raw huprot protein microarray matrices  (Mendeley Ltd)
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    Mendeley Ltd raw huprot protein microarray matrices
    Autoantibody Detection Unveils an Autoreactive Repertoire Enriched in MIS-C Patients (A) Upset plots delineating the number of shared autoantibodies between MIS-C patients, which were at least two-fold enriched when compared with controls for IgG autoantigens in <t>HuProt</t> protein <t>microarray</t> analysis. Upset plots were anchored on autoantibodies that were present in at least one IVIG-treatment-naïve sample (MIS-C 3 and MIS-C 9). Only intersections of 6 or more patients are visualized. (B) Corresponding upset plots for IgA autoantigens. (C) Heatmap of all IgG autoantigens with at least 4-fold enrichment in MIS-C compared to controls, in addition to the selection criteria above. Color intensity corresponds to the log 2 FC expression value relative to the mean of healthy pediatric controls (N=4). Flagged autoantigens were enriched in 5 patients and at least one treatment naive IVIG (MIS-C 3 or MIS-C 9) sample. (D) Corresponding heatmap for IgA autoantigens. (E) Top: validation of protein microarray hits identified by phage immunoprecipitation sequencing (PhIP-seq) for IgG autoantigens. The purple circle and corresponding number indicate the number of autoantigens enriched in the HuProt protein microarray that were also validated by PhIP-seq. Autoantigen peptides were collapsed at the gene level for overlap analyses. Bottom: corresponding overlap for IgA autoantigens. (F) Standard ELISA for CD244 auto-reactivity in MIS-C and healthy control plasma. (G) GSEA (gene set enrichment analysis) analysis of IgG autoantigens in treatment naïve MIS-C patients (N = 2; MIS-C 3 and MIS-C 9) versus age matched healthy controls (N = 4) ranked by t statistic. Dot color intensity corresponds to adjusted p value (FDR) and dot size represents the number of autoantigens found to be enriched in the associated gene set. (H) Corresponding enrichment scores for significantly (FDR<0.05) enriched biological pathways for IgG (regulation of immune response) and IgA (lymphocyte mediated immunity). Benjamini-Hochberg method was used to correct for multiple comparisons. See also <xref ref-type=Figure S5 . " width="250" height="auto" />
    Raw Huprot Protein Microarray Matrices, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/raw huprot protein microarray matrices/product/Mendeley Ltd
    Average 90 stars, based on 1 article reviews
    raw huprot protein microarray matrices - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Autoantibody Detection Unveils an Autoreactive Repertoire Enriched in MIS-C Patients (A) Upset plots delineating the number of shared autoantibodies between MIS-C patients, which were at least two-fold enriched when compared with controls for IgG autoantigens in HuProt protein microarray analysis. Upset plots were anchored on autoantibodies that were present in at least one IVIG-treatment-naïve sample (MIS-C 3 and MIS-C 9). Only intersections of 6 or more patients are visualized. (B) Corresponding upset plots for IgA autoantigens. (C) Heatmap of all IgG autoantigens with at least 4-fold enrichment in MIS-C compared to controls, in addition to the selection criteria above. Color intensity corresponds to the log 2 FC expression value relative to the mean of healthy pediatric controls (N=4). Flagged autoantigens were enriched in 5 patients and at least one treatment naive IVIG (MIS-C 3 or MIS-C 9) sample. (D) Corresponding heatmap for IgA autoantigens. (E) Top: validation of protein microarray hits identified by phage immunoprecipitation sequencing (PhIP-seq) for IgG autoantigens. The purple circle and corresponding number indicate the number of autoantigens enriched in the HuProt protein microarray that were also validated by PhIP-seq. Autoantigen peptides were collapsed at the gene level for overlap analyses. Bottom: corresponding overlap for IgA autoantigens. (F) Standard ELISA for CD244 auto-reactivity in MIS-C and healthy control plasma. (G) GSEA (gene set enrichment analysis) analysis of IgG autoantigens in treatment naïve MIS-C patients (N = 2; MIS-C 3 and MIS-C 9) versus age matched healthy controls (N = 4) ranked by t statistic. Dot color intensity corresponds to adjusted p value (FDR) and dot size represents the number of autoantigens found to be enriched in the associated gene set. (H) Corresponding enrichment scores for significantly (FDR<0.05) enriched biological pathways for IgG (regulation of immune response) and IgA (lymphocyte mediated immunity). Benjamini-Hochberg method was used to correct for multiple comparisons. See also <xref ref-type=Figure S5 . " width="100%" height="100%">

    Journal: Cell

    Article Title: Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C)

    doi: 10.1016/j.cell.2020.09.034

    Figure Lengend Snippet: Autoantibody Detection Unveils an Autoreactive Repertoire Enriched in MIS-C Patients (A) Upset plots delineating the number of shared autoantibodies between MIS-C patients, which were at least two-fold enriched when compared with controls for IgG autoantigens in HuProt protein microarray analysis. Upset plots were anchored on autoantibodies that were present in at least one IVIG-treatment-naïve sample (MIS-C 3 and MIS-C 9). Only intersections of 6 or more patients are visualized. (B) Corresponding upset plots for IgA autoantigens. (C) Heatmap of all IgG autoantigens with at least 4-fold enrichment in MIS-C compared to controls, in addition to the selection criteria above. Color intensity corresponds to the log 2 FC expression value relative to the mean of healthy pediatric controls (N=4). Flagged autoantigens were enriched in 5 patients and at least one treatment naive IVIG (MIS-C 3 or MIS-C 9) sample. (D) Corresponding heatmap for IgA autoantigens. (E) Top: validation of protein microarray hits identified by phage immunoprecipitation sequencing (PhIP-seq) for IgG autoantigens. The purple circle and corresponding number indicate the number of autoantigens enriched in the HuProt protein microarray that were also validated by PhIP-seq. Autoantigen peptides were collapsed at the gene level for overlap analyses. Bottom: corresponding overlap for IgA autoantigens. (F) Standard ELISA for CD244 auto-reactivity in MIS-C and healthy control plasma. (G) GSEA (gene set enrichment analysis) analysis of IgG autoantigens in treatment naïve MIS-C patients (N = 2; MIS-C 3 and MIS-C 9) versus age matched healthy controls (N = 4) ranked by t statistic. Dot color intensity corresponds to adjusted p value (FDR) and dot size represents the number of autoantigens found to be enriched in the associated gene set. (H) Corresponding enrichment scores for significantly (FDR<0.05) enriched biological pathways for IgG (regulation of immune response) and IgA (lymphocyte mediated immunity). Benjamini-Hochberg method was used to correct for multiple comparisons. See also Figure S5 .

    Article Snippet: Raw HuProt protein microarray matrices , This paper; Mendeley data , https://doi.org/10.17632/9kcv4fdy3s.1.

    Techniques: Immunopeptidomics, Microarray, Selection, Expressing, Biomarker Discovery, Immunoprecipitation, Sequencing, Enzyme-linked Immunosorbent Assay, Control, Clinical Proteomics

    Journal: Cell

    Article Title: Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C)

    doi: 10.1016/j.cell.2020.09.034

    Figure Lengend Snippet:

    Article Snippet: Raw HuProt protein microarray matrices , This paper; Mendeley data , https://doi.org/10.17632/9kcv4fdy3s.1.

    Techniques: Virus, Recombinant, Saline, Blocking Assay, Staining, Microarray, Software, Immunoprecipitation